© Messe Düsseldorf Bechergläser mit Hähnen
10:4011:05 18. Nov 2021
10:4011:05 18. Nov 2021 iCal myOrganizer
MEDICA LABMED FORUM

MLF: Rapid detection of SARS-CoV-2 by low volume real-time single tube reverse transcription recombinase polymerase amplification

Session Next-generation biosensing

The COVID-19 pandemic highlights the need for fast and simple assays for nucleic acid detection. As an isothermal alternative to RT-qPCR, we outline the development of a detection scheme for SARS-CoV-2 RNA based on reverse transcription recombinase polymerase amplification (RT-RPA) technology. RPA uses recombination proteins in combination with a DNA polymerase for rapid amplification of target DNA at a constant temperature (39–42 °C) within 10 to 20 minutes and can be monitored in real-time with fluorescent probes.