MLF: Disposable silicon-based electrochemical micro-qPCR

I will present our recent work on a disposable silicon-based integrated micro-qPCR technology (TriSilix) that can chemically-amplify and detect pathogen-specific sequences of nucleic acids quantitatively in real-time. Unlike other silicon-based technologies, TriSilix can be produced at wafer-scale (~30 cents/chip) in a standard laboratory; we have developed a series of methodologies based on metal-assisted chemical (wet) etching, electroplating, thermal bonding and laser-cutting to enable a cleanroom-free low-cost fabrication that does not require processing in an advanced semiconductor foundry.